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Addgene

CS2 human OAZ
(Plasmid #14036)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Vector type
    xenopus/mammalian/avian/zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OAZ
  • Species
    H. sapiens (human)
  • Entrez Gene
    ZNF423 (a.k.a. Ebfaz, JBTS19, NPHP14, OAZ, Roaz, ZFP423, Zfp104, hOAZ)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer EBV rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A human OAZ cDNA missing the first 18 codons (KIAA0760) was obtained from the Kazusa DNA Research Institute. A clone containing the amino-terminal end of human OAZ cDNA (ATCC Clone No. 2019478) was obtained from the EST database and sequenced. Full-length cDNA encoding human OAZ was constructed by fusing synthesized oligonucleotides encoding the first 18 amino acids to the KIAA0760 clone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CS2 human OAZ was a gift from Joan Massague (Addgene plasmid # 14036 ; http://n2t.net/addgene:14036 ; RRID:Addgene_14036)

  • For your References section:

    OAZ uses distinct DNA- and protein-binding zinc fingers in separate BMP-Smad and Olf signaling pathways. Hata A, Seoane J, Lagna G, Montalvo E, Hemmati-Brivanlou A, Massague J. Cell. 2000 Jan 21. 100(2):229-40. 10.1016/S0092-8674(00)81561-5 PubMed 10660046
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