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Addgene

pDEST40-2XFL-Src-S32-KD
(Plasmid #140311)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140311 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pcDNA-pDEST40
  • Backbone manufacturer
    modified from ThermoFisher 12274015
  • Backbone size w/o insert (bp) 7100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Src deletion mutant
  • Alt name
    S32-KD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1389
  • Mutation
    Contains only the SH3, SH2, and KD regions
  • Entrez Gene
    SRC (a.k.a. ASV, SRC1, THC6, c-SRC, p60-Src)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2X Flag (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST40-2XFL-Src-S32-KD was a gift from Mark Moasser (Addgene plasmid # 140311 ; http://n2t.net/addgene:140311 ; RRID:Addgene_140311)

  • For your References section:

    A Dimerization Function in the Intrinsically Disordered N-Terminal Region of Src. Spassov DS, Ruiz-Saenz A, Piple A, Moasser MM. Cell Rep. 2018 Oct 9;25(2):449-463.e4. doi: 10.1016/j.celrep.2018.09.035. 10.1016/j.celrep.2018.09.035 PubMed 30304684
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