pOP/SSAT
(Plasmid
#140291)
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PurposeExpresses human SSAT in bacterial cells
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 140291 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOP-SVI-MCS
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSSAT1
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SpeciesH. sapiens (human)
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Entrez GeneSAT1 (a.k.a. DC21, KFSD, KFSDX, SAT, SSAT, SSAT-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOP/SSAT was a gift from Saverio Bettuzzi & Gaetano Marverti (Addgene plasmid # 140291 ; http://n2t.net/addgene:140291 ; RRID:Addgene_140291) -
For your References section:
Spermidine/spermine N1-acetyltransferase transient overexpression restores sensitivity of resistant human ovarian cancer cells to N1,N12-bis(ethyl)spermine and to cisplatin. Marverti G, Giuseppina Monti M, Pegg AE, McCloskey DE, Bettuzzi S, Ligabue A, Caporali A, D'Arca D, Moruzzi MS. Carcinogenesis. 2005 Oct;26(10):1677-86. doi: 10.1093/carcin/bgi129. Epub 2005 May 19. 10.1093/carcin/bgi129 PubMed 15905201