pSLAX13 HA His (CT#244)
(Plasmid #14029)

• Purpose: (Empty Backbone)
• Depositing Lab: Cliff Tabin
• Publication: Logan et al Methods. 1998 Apr . 14(4):407-20.

Backbone

• Vector backbone: pSLAX13
• Backbone size (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • 3x HA
  • His

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector.

pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site.

For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html