pTAPmyc-T2A-tagRFP
(Plasmid
#140278)
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PurposeBackbone for making a mammalian expression vector of the protein of interest
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140278 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRES2 DsRed-Express
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Backbone manufacturerClontech
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Vector typeMammalian Expression, Synthetic Biology
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEmpty backbone
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SpeciesEmpty backbone
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Tag
/ Fusion Protein
- myc-T2A-tagRFP (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTAPmyc-T2A-tagRFP was a gift from Hirohide Saito (Addgene plasmid # 140278 ; http://n2t.net/addgene:140278 ; RRID:Addgene_140278) -
For your References section:
Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells. Kawasaki S, Fujita Y, Nagaike T, Tomita K, Saito H. Nucleic Acids Res. 2017 May 19. doi: 10.1093/nar/gkx298. 10.1093/nar/gkx298 PubMed 28525643
