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pAAV-F-RAM-d2tTA-TRE-mKate2
(Plasmid #140274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140274 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Derived from AAV V032
  • Total vector size (bp) 8368
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mKate2
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • Promoter TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Age1 (not destroyed)
  • 3′ cloning site Asc1 (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-F-RAM-d2tTA-TRE-mKate2 was a gift from Yingxi Lin (Addgene plasmid # 140274 ; http://n2t.net/addgene:140274 ; RRID:Addgene_140274)

  • For your References section:

    Functionally Distinct Neuronal Ensembles within the Memory Engram. Sun X, Bernstein MJ, Meng M, Rao S, Sorensen AT, Yao L, Zhang X, Anikeeva PO, Lin Y. Cell. 2020 Apr 16;181(2):410-423.e17. doi: 10.1016/j.cell.2020.02.055. Epub 2020 Mar 17. 10.1016/j.cell.2020.02.055 PubMed 32187527
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