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Addgene

pBoxCDGC-KMet-EGFP
(Plasmid #140272)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140272 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BoxCD
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer CTTTATTTGTAACCATTATAAGCTGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternative name: p1-boxC/D-EGFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBoxCDGC-KMet-EGFP was a gift from Hirohide Saito (Addgene plasmid # 140272 ; http://n2t.net/addgene:140272 ; RRID:Addgene_140272)

  • For your References section:

    Synthetic translational regulation by an L7Ae-kink-turn RNP switch. Saito H, Kobayashi T, Hara T, Fujita Y, Hayashi K, Furushima R, Inoue T. Nat Chem Biol. 2010 Jan;6(1):71-8. doi: 10.1038/nchembio.273. Epub 2009 Dec 13. 10.1038/nchembio.273 PubMed 20016495
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