pU1A-myc-T2A-tagRFP
(Plasmid #140269)

• Purpose: Expresses truncated U1A in mammalian cells
• Depositing Lab: Hirohide Saito
• Publication: Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.1093/nar/gkx298.

Backbone

• Vector backbone: pIRES2 DsRed-Express
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Truncated U1A
• Species: H. sapiens (human)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc-T2A-tagRFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: CTTTATTTGTAACCATTATAAGCTGC

Resource Information

• Supplemental Documents:
  • pU1A-myc-T2A-tagRFP.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CDS of Truncated U1A was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).