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Addgene

LCB7_crtYB
(Plasmid #140232)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140232 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LCB7
  • Total vector size (bp) 4859
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phytoene-beta carotene synthase
  • Alt name
    crtYB
  • Insert Size (bp)
    2016
  • Mutation
    K307E
  • GenBank ID
    AII26676.1
  • Promoter pthrC3

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TagcaatctaatctaagttttctagaaaaatG
  • 3′ sequencing primer CttttagtgatggtgatgatgatgttA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The minor differences between the sequence and .gb file are of no functional concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LCB7_crtYB was a gift from Kang Zhou (Addgene plasmid # 140232 ; http://n2t.net/addgene:140232 ; RRID:Addgene_140232)
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