Junjie Chen Lab DNA Damage Response MKOv4 Library
(Pooled Library #140219)

• Purpose:

  This lentiviral CRISPR knockout library targets human genes with known or suspected roles in DNA damage response and DNA repair.

• Vector Backbone:

  lentiCRISPR v2 (Plasmid #52961) - expresses Cas9

• Depositing Labs: Junjie Chen
• Publication: Su et al DNA Repair (Amst). 2020 Jan 16;87:102803. doi: 10.1016/j.dnarep.2020.102803.

Library Details

• Species: Human
• Genes targeted:
  
  • 365 DDR genes
  • 45 core-essential genes
  • 50 non-essential genes
• gRNAs: 4,530
• Controls:
  
  • 443 positive control sgRNAs
  • 500 negative control sgRNAs
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Transformation and Screening Protocols (PDF, 250 KB)
• Depositor Data:
  • List of Targeted Genes and gRNA Sequences (XLSX, 300 KB)
  • Representative NGS Results (XLSX, 119 KB)
• Articles Citing this Pooled Library:
  • DDR MKOv4 (#140219)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms for analysis.