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Purpose(Empty Backbone) Lenti Crispr/Cas9 with mOrange fluorescent marker
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140206 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLentiCrispr-V2-mOrange (V2mO)
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Backbone manufacturerMIT
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Modifications to backboneInserted mOrange into pLentiCrisprV2 to make V2mO.
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Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter pELF1a
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Selectable markersPuromycin ; mOrange flurescent marker
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsStabl2, Stabl3
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Lenti Crispr/Cas9 V2 with mOrange fluorescent marker, to make lentiviral production visible and target cell transduction visible with mOrange.
The cloning method for inserting a guide RNA (gRNA) is: BsmB1.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLentiCrispr-V2-mOrange (V2mO) was a gift from Jaffer Ajani (Addgene plasmid # 140206 ; http://n2t.net/addgene:140206 ; RRID:Addgene_140206) -
For your References section:
An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue. Jin J, Xu Y, Huo L, Ma L, Scott AW, Pizzi MP, Li Y, Wang Y, Yao X, Song S, Ajani JA. PLoS One. 2020 Feb 13;15(2):e0228910. doi: 10.1371/journal.pone.0228910. eCollection 2020. 10.1371/journal.pone.0228910 PubMed 32053639
Map uploaded by the depositor.
