Skip to main content
Addgene

pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS
(Plasmid #140196)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGem
  • Backbone size w/o insert (bp) 3033
  • Total vector size (bp) 12892
  • Vector type
    CRISPR, Synthetic Biology ; Fungal genome integration.
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    dSpCas9-VPR
  • Alt name
    dCas9-VPR
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    5730
  • Promoter PgpdA
  • Tag / Fusion Protein
    • VPR (VP64-p65-Rta) , NLS (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Bar
  • Alt name
    Glufosinate/basta-resistance
  • Species
    Streptomyces hygroscopicus
  • Insert Size (bp)
    552
  • Promoter PtrpC

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
  • Species
    Aspergillus nidulans
  • Insert Size (bp)
    1040

Gene/Insert 4

  • Gene/Insert name
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
  • Species
    Aspergillus nidulans
  • Insert Size (bp)
    1033

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To be linerised with NotI for homologous recombination in Aspergillus nidulans nkuA deficient strains, selecting with glufosinate/Basta. It can also be used for random genome integration in other fungal strains.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS was a gift from Yit Heng Chooi (Addgene plasmid # 140196 ; http://n2t.net/addgene:140196 ; RRID:Addgene_140196)

  • For your References section:

    CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi. Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH. ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197. 10.1021/acssynbio.0c00197 PubMed 32526136
Related items: