-
PurposeOverexpression of Progerin with a GFP fusion protein, under human synapsin promotor
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 140189 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLenti-hSyn
- Total vector size (bp) 11092
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameProgerin
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1845
-
Entrez GeneLMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
-
Tag
/ Fusion Protein
- GFP
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLenti-hSyn-PROGERIN was a gift from Lorenz Studer (Addgene plasmid # 140189 ; http://n2t.net/addgene:140189 ; RRID:Addgene_140189) -
For your References section:
Human iPSC-based modeling of late-onset disease via progerin-induced aging. Miller JD, Ganat YM, Kishinevsky S, Bowman RL, Liu B, Tu EY, Mandal PK, Vera E, Shim JW, Kriks S, Taldone T, Fusaki N, Tomishima MJ, Krainc D, Milner TA, Rossi DJ, Studer L. Cell Stem Cell. 2013 Dec 5;13(6):691-705. doi: 10.1016/j.stem.2013.11.006. 10.1016/j.stem.2013.11.006 PubMed 24315443