pEF-pIgH-ESCargo*
(Plasmid
#140159)
-
PurposeExpresses a regulatable secretory cargo (without an ER export signal) for mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140159 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEF/myc/ER
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namepiGH-DsRed-Express2-FKBP(LV)(C22V)
-
SpeciesSynthetic
- Promoter EF-1a
-
Tag
/ Fusion Protein
- ER signal sequence (MGWSCIILFLVATATGAHS) (N terminal on backbone) (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEF-pIgH-ESCargo* was a gift from Benjamin Glick (Addgene plasmid # 140159 ; http://n2t.net/addgene:140159 ; RRID:Addgene_140159) -
For your References section:
ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms. Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz AP, Horne-Badovinac S, Green WN, Glick BS. Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591. 10.1091/mbc.E20-09-0591 PubMed 33112725
