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Addgene

YIplac211-Sec7-mScarlet2Ix6
(Plasmid #140151)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YIplac211
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SEC7
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Parental strain JK93da.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YIplac211-Sec7-mScarlet2Ix6 was a gift from Benjamin Glick (Addgene plasmid # 140151 ; http://n2t.net/addgene:140151 ; RRID:Addgene_140151)

  • For your References section:

    A microscopy-based kinetic analysis of yeast vacuolar protein sorting. Casler JC, Glick BS. Elife. 2020 Jun 25;9. pii: 56844. doi: 10.7554/eLife.56844. 10.7554/eLife.56844 PubMed 32584255
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