YIplac211-Vph1-HaloTag
(Plasmid
#140149)
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PurposePlasmid for pop-in/pop-out replacement of Vph1 with Vph1-HaloTag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneYIplac211
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlease grow for 16-20 hours
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVPH1
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Parental strain JK93da.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YIplac211-Vph1-HaloTag was a gift from Benjamin Glick (Addgene plasmid # 140149 ; http://n2t.net/addgene:140149 ; RRID:Addgene_140149) -
For your References section:
A microscopy-based kinetic analysis of yeast vacuolar protein sorting. Casler JC, Glick BS. Elife. 2020 Jun 25;9. pii: 56844. doi: 10.7554/eLife.56844. 10.7554/eLife.56844 PubMed 32584255
