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Addgene

pYFP-lacY-10
(Plasmid #140139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAN1818
  • Backbone size w/o insert (bp) 4938
  • Total vector size (bp) 6241
  • Modifications to backbone
    RBS of encoded lacI is changed to increase translation ~10-fold
  • Vector type
    Bacterial Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MG1655dlac
  • Growth instructions
    Zeocin resistant
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    lacY
  • Alt name
    lactose permease
  • Insert Size (bp)
    1254
  • Entrez Gene
    lacY (a.k.a. b0343, ECK0340)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATCCGCCACAACATCGAGGACGGCAGCGTGCAG
  • 3′ sequencing primer TCCGCCATCGCCGCTTCCACTTTTTCCCGCGTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYFP-lacY-10 was a gift from Drew Tack (Addgene plasmid # 140139 ; http://n2t.net/addgene:140139 ; RRID:Addgene_140139)

  • For your References section:

    Sparse estimation of mutual information landscapes quantifies information transmission through cellular biochemical reaction networks. Sarkar S, Tack D, Ross D. Commun Biol. 2020 Apr 30;3(1):203. doi: 10.1038/s42003-020-0901-9. 10.1038/s42003-020-0901-9 PubMed 32355194
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