pYFP-lacY-1
(Plasmid #140138)

• Purpose: Evaluating the impact of feedback on information transmission of a genetic sensor.
• Depositing Lab: Drew Tack
• Publication: Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi: 10.1038/s42003-020-0901-9.

Backbone

• Vector backbone: pAN1818
• Backbone size w/o insert (bp): 4938
• Total vector size (bp): 6241
• Vector type: Bacterial Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): MG1655dlac
• Growth instructions: Zeocin resistant
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: lacY
• Alt name: lactose permease
• Insert Size (bp): 1254
• Entrez Gene: lacY (a.k.a. b0343, ECK0340)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATCCGCCACAACATCGAGGACGGCAGCGTGCAG
• 3′ sequencing primer: AACGCGGGAAAAAGTGGAAGCGGCGATGGCGGA

Resource Information

• Supplemental Documents:
  • pYFP-lacY.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.