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Addgene

pHis8-4b-McDAH
(Plasmid #140129)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140129 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHis8-4b
  • Backbone size w/o insert (bp) 5276
  • Total vector size (bp) 6332
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dechloroacutumine halogenase from Menispermum canadense
  • Alt name
    McDAH
  • Species
    Menispermum canadense
  • Insert Size (bp)
    1056
  • GenBank ID
    MT040709
  • Promoter T7
  • Tag / Fusion Protein
    • 8x-His, TEV protease site (ENLYFQ) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expressed robustly in E.coli. Catalyzes chlorination of dechloroacutumine in vitro

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHis8-4b-McDAH was a gift from Jing-Ke Weng (Addgene plasmid # 140129 ; http://n2t.net/addgene:140129 ; RRID:Addgene_140129)

  • For your References section:

    The chloroalkaloid (-)-acutumine is biosynthesized via a Fe(II)- and 2-oxoglutarate-dependent halogenase in Menispermaceae plants. Kim CY, Mitchell AJ, Glinkerman CM, Li FS, Pluskal T, Weng JK. Nat Commun. 2020 Apr 20;11(1):1867. doi: 10.1038/s41467-020-15777-w. 10.1038/s41467-020-15777-w PubMed 32313070
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