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Addgene

pDECKO_mCherry_TREML2
(Plasmid #140106)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140106 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDECKO_mCherry
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA1+scaffold+H1promoter+gRNA2
  • gRNA/shRNA sequence
    2 guide RNAs againsts lncRNA TREML2
  • Species
    H. sapiens (human)
  • Promoter gRNA1 under U6 and gRNA2 under H1

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDECKO_mCherry_TREML2 was a gift from Roderic Guigo (Addgene plasmid # 140106 ; http://n2t.net/addgene:140106 ; RRID:Addgene_140106)

  • For your References section:

    Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages. Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo R. BMC Genomics. 2022 May 26;23(1):402. doi: 10.1186/s12864-022-08612-7. 10.1186/s12864-022-08612-7 PubMed 35619054
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