pCAG-BE4max-P2A-EGFP (pRZ33)
(Plasmid
#140083)
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PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140083 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAG backbone
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Backbone manufacturerJoung Lab (SQT817, Addgene #53373)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman codon optimized pCAG BE4max with P2A-EGFP
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Alt namepRZ33
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SpeciesSynthetic
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MutationD10A (SpCas9)
- Promoter CAG
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Tag
/ Fusion Protein
- P2A-EGFP (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer oBK280-CCTACAGCTCCTGGGCAACG
- 3′ sequencing primer oBK23-CAGAGGGAAAAAGATCTCAGTGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
BE4max from Addgene #112093; P2A-EGFP from BPK4335
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-BE4max-P2A-EGFP (pRZ33) was a gift from Keith Joung (Addgene plasmid # 140083 ; http://n2t.net/addgene:140083 ; RRID:Addgene_140083)
Map uploaded by the depositor.
