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pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
(Plasmid #140082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    DR274 (Addgene Plasmid #42250)
  • Backbone manufacturer
    Joung Lab (DR274)
  • Vector type
    Bacterial Expression ; In vitro transcription (mRNA synthesis)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
  • Alt name
    MSP3485
  • gRNA/shRNA sequence
    GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC (this is the sequence of the SpCas9 sgRNA scaffold)
  • Species
    Synthetic
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer oBK7349-GTAAGATCACGGGTCGCAGCAC
  • 3′ sequencing primer M13F-TGTAAAACGACGGCCAGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485) was a gift from Keith Joung (Addgene plasmid # 140082 ; http://n2t.net/addgene:140082 ; RRID:Addgene_140082)
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