pcDNA3-ABKAR
(Plasmid #140051)

• Purpose: Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.
• Depositing Lab: Jin Zhang
• Publication: Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-46. doi: 10.1091/mbc.E14-02-0764. Epub 2015 Mar 18.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5517
• Total vector size (bp): 7494
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ABKAR
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 1977
• Promoter: CMV
• Tags / Fusion Proteins:
  • Cerulean3 (N terminal on insert)
  • cpVenus[E172] (C terminal on insert)
  • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-ABKAR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry