pGuide-P1-O1a (EL702)
(Plasmid
#140040)
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PurposeCRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGuide
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebarcode P1 and sgRNA lacO1 array
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gRNA/shRNA sequencegRNA scaffold
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGuide-P1-O1a (EL702) was a gift from Johan Elf (Addgene plasmid # 140040 ; http://n2t.net/addgene:140040 ; RRID:Addgene_140040) -
For your References section:
Time-resolved imaging-based CRISPRi screening. Camsund D, Lawson MJ, Larsson J, Jones D, Zikrin S, Fange D, Elf J. Nat Methods. 2020 Jan;17(1):86-92. doi: 10.1038/s41592-019-0629-y. Epub 2019 Nov 18. 10.1038/s41592-019-0629-y PubMed 31740817
