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Addgene

pGuide-P1-O1a (EL702)
(Plasmid #140040)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140040 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGuide
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    barcode P1 and sgRNA lacO1 array
  • gRNA/shRNA sequence
    gRNA scaffold

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGuide-P1-O1a (EL702) was a gift from Johan Elf (Addgene plasmid # 140040 ; http://n2t.net/addgene:140040 ; RRID:Addgene_140040)

  • For your References section:

    Time-resolved imaging-based CRISPRi screening. Camsund D, Lawson MJ, Larsson J, Jones D, Zikrin S, Fange D, Elf J. Nat Methods. 2020 Jan;17(1):86-92. doi: 10.1038/s41592-019-0629-y. Epub 2019 Nov 18. 10.1038/s41592-019-0629-y PubMed 31740817
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