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Addgene

pSW036
(Plasmid #140034)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140034 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Total vector size (bp) 5144
  • Modifications to backbone
    Up and downstream homology regions for recombination into the acsA locus in PCC 11901; cpt promoter
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Yellow fluorescent protein
  • Alt name
    YFP
  • Species
    Synthetic; Aequorea victoria
  • Insert Size (bp)
    801
  • GenBank ID
    JX472996.1
  • Promoter cpt

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aggtcatatccgaggcgtacattca
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/684944v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSW036 was a gift from Peter Nixon (Addgene plasmid # 140034 ; http://n2t.net/addgene:140034 ; RRID:Addgene_140034)

  • For your References section:

    Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production. Wlodarczyk A, Selao TT, Norling B, Nixon PJ. Commun Biol. 2020 May 7;3(1):215. doi: 10.1038/s42003-020-0910-8. 10.1038/s42003-020-0910-8 PubMed 32382027
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