pSZT025
(Plasmid #140033)

• Purpose: KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter
• Depositing Lab: Peter Nixon
• Publication: Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 10.1038/s42003-020-0910-8.

Backbone

• Vector backbone: pUC19
• Total vector size (bp): 6707
• Modifications to backbone: PCC 7002 fadD up and downstream sequences for homologous recombination; Kanamycin resistance cassette; cLac143 promoter and LacI gene under pMB2 promoter control
• Vector type: Bacterial Expression
• Selectable markers: Ampicillin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Truncated thioesterase gene
• Alt name: 'tesA
• Species: Escherichia coli
• Insert Size (bp): 549
• Promoter: cLac143

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGATGGGTGAAATCTGGTTTTAAAGTAGG
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pszt025-puc19_delta_fadd_clac143_ec-tesa_wt_kmr.gb
• A portion of this plasmid was derived from a plasmid made by: GenScript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/684944v1 for bioRxiv preprint.