XLone-eGFP NLS (no drug resistant gene)
(Plasmid
#140032)
-
PurposeTunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140032 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneAddgene #96930
- Backbone size w/o insert (bp) 5874
- Total vector size (bp) 6591
-
Modifications to backboneblasticidin resistant gene switched to eGFP
-
Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEnhanced Green Fluorescent Protein
-
Alt nameeGFP
-
SpeciesSynthetic
- Promoter TRE3G
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer M13 FWD
- 3′ sequencing primer M13 RVS (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
XLone-eGFP NLS (no drug resistant gene) was a gift from Xiaoping Bao (Addgene plasmid # 140032 ; http://n2t.net/addgene:140032 ; RRID:Addgene_140032)
Map uploaded by the depositor.
