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XLone-BSD NFIL3 T2A Puro
(Plasmid #140028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene #96930
  • Backbone size w/o insert (bp) 5607
  • Total vector size (bp) 7688
  • Modifications to backbone
    eGFP switched to NFIL3-T2A-Puro
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Puromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFIL3
  • Alt name
    E4BP4
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1386
  • Entrez Gene
    NFIL3 (a.k.a. E4BP4, IL3BP1, NF-IL3A, NFIL3A)
  • Promoter TRE3G
  • Tag / Fusion Protein
    • puromycin (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NheI or SpeI (not destroyed)
  • 5′ sequencing primer M13 FWD
  • 3′ sequencing primer M13 RVS
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XLone-BSD NFIL3 T2A Puro was a gift from Xiaoping Bao (Addgene plasmid # 140028 ; http://n2t.net/addgene:140028 ; RRID:Addgene_140028)
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