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RCAS(A) dRhoA N19 (CT#847)
(Plasmid #14002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14002 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RhoA
  • Alt name
    RhoA-N19
  • Alt name
    Dominant negative RhoA
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    600
  • Mutation
    T19N
  • Entrez Gene
    Rho1 (a.k.a. Dmel_CG8416, 19549712, 8416, AAF01186, CG8416, D-Rho1, DRho, DRho1, DRhoA, Dm Rho1, DmRHO-A, Dmel\CG8416, Drho1, DrhoA, E3.10/J3.8, P48148, RHO, RHO1, RHOA, Rho, Rho A, Rho GTPase, Rho kinase, RhoA, RhoN19, dRho1, dRhoA, dnRho, l(2)52Fa, l(2)k02107b, n(2)k07236, rho, rho1, rhoA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS(A) dRhoA N19 (CT#847) was a gift from Cliff Tabin (Addgene plasmid # 14002 ; http://n2t.net/addgene:14002 ; RRID:Addgene_14002)

  • For your References section:

    Wnt-14 plays a pivotal role in inducing synovial joint formation in the developing appendicular skeleton. Hartmann C, Tabin CJ. Cell. 2001 Feb 9. 104(3):341-51. 10.1016/S0092-8674(01)00222-7 PubMed 11239392
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