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pAS_4xMx PylT_GFP 150TAG
(Plasmid #140016)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140016 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Alt name
    4xh7SK-Mx1201 PylT cassette
  • Species
    Aequorea victoria
  • Insert Size (bp)
    750
  • Mutation
    150TAG, K46E (Please see depositor comments below)
  • Promoter EF1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms K46E point mutation in sfGFP 150 tag does not affect GFP fluorescence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_4xMx PylT_GFP 150TAG was a gift from Simon Elsaesser (Addgene plasmid # 140016 ; http://n2t.net/addgene:140016 ; RRID:Addgene_140016)

  • For your References section:

    Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells. Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ. ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571. 10.1021/acschembio.8b00571 PubMed 30260624
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