pAS_4xMma PylT(UUA)_FLAG-AcKRS
(Plasmid #140010)

• Purpose: Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration
• Depositing Lab: Simon Elsaesser
• Publication: Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.

Backbone

• Vector backbone: pAS
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AcKRS-3
• Alt name: 4xh7SK-Mma PylT (TAA) cassette
• Species: Methanosarcina mazei
• Insert Size (bp): 1412
• Mutation: L301M, L305I, Y306F, L309A, C348F
• Entrez Gene: pylS (a.k.a. MSMAC_RS17105, MSMAC_3241)
• Promoter: EF1
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • pas_4xmmapyltuua_flag-ackrs.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there are several ambiguous bases in the 2 small regions in the h7SK promoter and PylT TAA repeats in Addgene's NGS result. Depositor has confirmed plasmid is functional.