pEarleyGate103 mmPing20F
(Plasmid
#140007)
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PurposemmPing20F activation tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140007 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEarleyGate 103
- Total vector size (bp) 12603
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Vector typePlant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemmPing20F
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SpeciesOryza sativa and Fig Mosaic Virus
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer 35S For
- 3′ sequencing primer GFP Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid contains an IS element 3' of the KanR KanR marker that is not currently of functional concern. Confirm IS element remains in expected position prior to use or when troubleshooting.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEarleyGate103 mmPing20F was a gift from Charles Nathan Hancock (Addgene plasmid # 140007 ; http://n2t.net/addgene:140007 ; RRID:Addgene_140007) -
For your References section:
Development of mPing-based activation tags for crop insertional mutagenesis. Johnson A, Mcassey E, Diaz S, Reagin J, Redd PS, Parrilla DR, Nguyen H, Stec A, McDaniel LAL, Clemente TE, Stupar RM, Parrott WA, Hancock CN. Plant Direct. 2021 Jan 19;5(1):e00300. doi: 10.1002/pld3.300. eCollection 2021 Jan. 10.1002/pld3.300 PubMed 33506165