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Addgene

pWMD23
(Plasmid #140006)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140006 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCambia
  • Backbone manufacturer
    CSIRO
  • Vector type
    Plant Expression
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ORF1 Shuffle 1 NLS
  • Species
    Oryza sativa
  • Mutation
    Ping N-terminal, Pong C terminal with stronger NLS

Gene/Insert 2

  • Gene/Insert name
    Pong TPase LA
  • Species
    Oryza sativa
  • Mutation
    NES sequence mutated L418A, L420A

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid contains an IS element 5' of the KanR marker that is not currently of functional concern. Confirm IS element remains in expected position prior to use or when troubleshooting.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWMD23 was a gift from Charles Nathan Hancock (Addgene plasmid # 140006 ; http://n2t.net/addgene:140006 ; RRID:Addgene_140006)

  • For your References section:

    Development of mPing-based activation tags for crop insertional mutagenesis. Johnson A, Mcassey E, Diaz S, Reagin J, Redd PS, Parrilla DR, Nguyen H, Stec A, McDaniel LAL, Clemente TE, Stupar RM, Parrott WA, Hancock CN. Plant Direct. 2021 Jan 19;5(1):e00300. doi: 10.1002/pld3.300. eCollection 2021 Jan. 10.1002/pld3.300 PubMed 33506165
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