pWMD23
(Plasmid #140006)

• Purpose: Expression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)
• Depositing Lab: Charles Nathan Hancock
• Publication: Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. doi: 10.1002/pld3.300. eCollection 2021 Jan.

Backbone

• Vector backbone: pCambia
• Backbone manufacturer: CSIRO
• Vector type: Plant Expression
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ORF1 Shuffle 1 NLS
• Species: Oryza sativa
• Mutation: Ping N-terminal, Pong C terminal with stronger NLS

Gene/Insert 2

• Gene/Insert name: Pong TPase LA
• Species: Oryza sativa
• Mutation: NES sequence mutated L418A, L420A

Resource Information

• Supplemental Documents:
  • pWMD23.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid contains an IS element 5' of the KanR marker that is not currently of functional concern. Confirm IS element remains in expected position prior to use or when troubleshooting.