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Addgene

pBS mShh (CT#258)
(Plasmid #13999)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13999 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS SK
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Shh
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2600
  • Entrez Gene
    Shh (a.k.a. 9530036O11Rik, Dsh, HHG-1, Hhg1, Hx, Hxl3, M100081, ShhNC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full length (~250bp 5’ UTR, 1314bp coding, plus ~1kbp 3’ UTR), from Andy McMahon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS mShh (CT#258) was a gift from Cliff Tabin (Addgene plasmid # 13999 ; http://n2t.net/addgene:13999 ; RRID:Addgene_13999)
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