pCAG/hTAZ-FL
(Plasmid #139979)

• Purpose: Expresses full length human Tafazzin with codon modified for cloning. Transgene flanked by AAV2 ITR
• Depositing Lab: William Pu
• Publication: Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCRESAHA.119.315956.

Want a viral vector made from this plasmid?

Make a packaging request and we'll get back to you.

This service is available to academics and nonprofits only.

Please log in to submit a packaging request.

• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
• How this works:
  • Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
  • Addgene will quickly confirm that we can produce a high-quality prep for you.
  • Track your request and place an order from within your account. Payment information must be added before we can begin processing your order.
  • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
  • Learn more about our Packaged on Request Service.

Backbone

• Vector backbone: AAV2/9.cTnT.PI.EGFP.RBG
• Backbone manufacturer: Penn Vector Core
• Backbone size w/o insert (bp): 4187
• Total vector size (bp): 5859
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Full length human Tafazzin
• Species: H. sapiens (human)
• Insert Size (bp): 887
• GenBank ID: NM_000116.5
• Entrez Gene: TAFAZZIN (a.k.a. BTHS, CMD3A, EFE, EFE2, G4.5, LVNCX, TAZ, Taz1)
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AGEI (unknown if destroyed)
• 3′ cloning site: HINDIII (unknown if destroyed)
• 5′ sequencing primer: GATGCCTCTGCACGTGAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry