EPHB4-gRNA1/2
(Plasmid
#139974)
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PurposegRNA used for knockin EGFP into EPHB4 allele
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSTBlue-1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEPHB4 gRNA
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gRNA/shRNA sequenceCCCCGCAGTACTGACCTGC
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EPHB4-gRNA1/2 was a gift from James Thomson (Addgene plasmid # 139974 ; http://n2t.net/addgene:139974 ; RRID:Addgene_139974) -
For your References section:
Functional characterization of human pluripotent stem cell-derived arterial endothelial cells. Zhang J, Chu LF, Hou Z, Schwartz MP, Hacker T, Vickerman V, Swanson S, Leng N, Nguyen BK, Elwell A, Bolin J, Brown ME, Stewart R, Burlingham WJ, Murphy WL, Thomson JA. Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):E6072-E6078. doi: 10.1073/pnas.1702295114. Epub 2017 Jul 10. 10.1073/pnas.1702295114 PubMed 28696312