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Addgene

EFNB2-gRNA3/4
(Plasmid #139973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSTBlue-1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EFNB2-gRNA
  • gRNA/shRNA sequence
    TGTAGTAAATGTTCGCCGG
  • Species
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EFNB2-gRNA3/4 was a gift from James Thomson (Addgene plasmid # 139973 ; http://n2t.net/addgene:139973 ; RRID:Addgene_139973)

  • For your References section:

    Functional characterization of human pluripotent stem cell-derived arterial endothelial cells. Zhang J, Chu LF, Hou Z, Schwartz MP, Hacker T, Vickerman V, Swanson S, Leng N, Nguyen BK, Elwell A, Bolin J, Brown ME, Stewart R, Burlingham WJ, Murphy WL, Thomson JA. Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):E6072-E6078. doi: 10.1073/pnas.1702295114. Epub 2017 Jul 10. 10.1073/pnas.1702295114 PubMed 28696312
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