pBS cIhh (CT#431)
(Plasmid
#13997)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13997 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBS SK-
- Backbone size w/o insert (bp) 3000
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameIhh
-
SpeciesG. gallus (chicken)
-
Insert Size (bp)1680
-
Entrez GeneIHH
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Total coding sequence plus 327bp 5’ UTR and 130bp 3’ UTR, from Andrea Vortkamp.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBS cIhh (CT#431) was a gift from Cliff Tabin (Addgene plasmid # 13997 ; http://n2t.net/addgene:13997 ; RRID:Addgene_13997) -
For your References section:
Regulation of rate of cartilage differentiation by Indian hedgehog and PTH-related protein. Vortkamp A, Lee K, Lanske B, Segre GV, Kronenberg HM, Tabin CJ. Science. 1996 Aug 2. 273(5275):613-22. 10.1126/science.273.5275.613 PubMed 8662546