pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG
(Plasmid
#139964)
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PurposeOver-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139964 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAMBIA-pBR322
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202
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SpeciesMultiple
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG was a gift from Raffaela Abbriano (Addgene plasmid # 139964 ; http://n2t.net/addgene:139964 ; RRID:Addgene_139964) -
For your References section:
Overexpression of Key Sterol Pathway Enzymes in Two Model Marine Diatoms Alters Sterol Profiles in Phaeodactylum tricornutum. Jaramillo-Madrid AC, Abbriano R, Ashworth J, Fabris M, Pernice M, Ralph PJ. Pharmaceuticals (Basel). 2020 Dec 21;13(12). pii: ph13120481. doi: 10.3390/ph13120481. 10.3390/ph13120481 PubMed 33371196