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Addgene

pBEL1731
(Plasmid #139922)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139922 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LetB
  • Species
    E. coli
  • Mutation
    LetB(43-877; E854Amber)-6xHis

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEL1731 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 139922 ; http://n2t.net/addgene:139922 ; RRID:Addgene_139922)

  • For your References section:

    LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope. Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G. Cell. 2020 Apr 30;181(3):653-664.e19. doi: 10.1016/j.cell.2020.03.030. 10.1016/j.cell.2020.03.030 PubMed 32359438
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