RCAS (E) shh (CT#489)
(Plasmid
#13992)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRCASBP(E)
- Backbone size w/o insert (bp) 11600
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Vector typeRetroviral ; avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameshh
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Alt namesonic hedgehog
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SpeciesG. gallus (chicken)
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Insert Size (bp)1500
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Entrez GeneSHH (a.k.a. ShhNC)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS (E) shh (CT#489) was a gift from Cliff Tabin (Addgene plasmid # 13992 ; http://n2t.net/addgene:13992 ; RRID:Addgene_13992) -
For your References section:
Sonic hedgehog mediates the polarizing activity of the ZPA. Riddle RD, Johnson RL, Laufer E, Tabin C. Cell. 1993 Dec 31. 75(7):1401-16. 10.1016/0092-8674(93)90626-2 PubMed 8269518
Map uploaded by the depositor.