pmRFP-SUMO1 GG
(Plasmid #13990)

• Depositing Lab: Jonathan Chernoff
• Publication: Dadke et al Nat Cell Biol. 2007 Jan . 9(1):80-85.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO1 GG
• Alt name: SUMO1
• Species: M. musculus (mouse)
• Insert Size (bp): 297
• Mutation: C-terminal truncation. Cannot ligate to substrates. Useful as negative control in sumoylation studies.
• Entrez Gene: Sumo1 (a.k.a. GMP1, PIC1, SENTRIN, SMT3, SMT3H3, SMTP3, SUMO-1, Smt3C, Ubl1)
• Tag / Fusion Protein:
  • mRFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: DsRed1-C

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry