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TetOn-REEP5-mCherry-eGFP
(Plasmid #139870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TetOn
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    REEP5
  • Species
    H. sapiens (human)
  • Entrez Gene
    REEP5 (a.k.a. C5orf18, D5S346, DP1, POB16, TB2, YOP1, Yip2e)
  • Tag / Fusion Protein
    • mCherry-eGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains L26P and V92E mutations in REEP5. These mutations are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TetOn-REEP5-mCherry-eGFP was a gift from Jacob Corn (Addgene plasmid # 139870 ; http://n2t.net/addgene:139870 ; RRID:Addgene_139870)

  • For your References section:

    A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation. Liang JR, Lingeman E, Luong T, Ahmed S, Muhar M, Nguyen T, Olzmann JA, Corn JE. Cell. 2020 Mar 19;180(6):1160-1177.e20. doi: 10.1016/j.cell.2020.02.017. Epub 2020 Mar 10. 10.1016/j.cell.2020.02.017 PubMed 32160526
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