RCAS (A) Hoxc6 (CT#541)
(Plasmid
#13986)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13986 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRCASBP(A)
- Backbone size w/o insert (bp) 11600
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Vector typeRetroviral ; avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHoxc6
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Alt nameHox C6
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Alt nameHoxc-6
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Alt namehomeobox c6
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SpeciesG. gallus (chicken)
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Insert Size (bp)900
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Entrez GeneHOXC6 (a.k.a. HOXC-6)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To generate a retrovirus capable of transcribing Hoxc-6 mRNA, the region encoding the PR11 Hoxc-6 protein was isolated from a chick cDNA clone (Nelson et al., 1996). The PR11 Hoxc-6 protein comprises 235 amino acids (Oliver et al., 1988; Coletta et al., 1991; Shimeld et al., 1993). The ATG of the PR11 coding region was engineered by PCR for cloning into the NcoI site of the SLAX-13 shuttle vector (Riddle et al., 1993), and then subcloned into the retroviral vector RCAS-BP(A) (Hughes et al., 1987). The insert is 932 base pairs and includes approximately 230 bp of 3' untranslated sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS (A) Hoxc6 (CT#541) was a gift from Cliff Tabin (Addgene plasmid # 13986 ; http://n2t.net/addgene:13986 ; RRID:Addgene_13986) -
For your References section:
Virally mediated misexpression of Hoxc-6 in the cervical mesoderm results in spinal nerve truncations. Burke AC, Tabin CJ. Dev Biol. 1996 Aug 25. 178(1):192-7. 10.1006/dbio.1996.0210 PubMed 8812121