RCAS (A) Hoxc6 (CT#541)
(Plasmid #13986)

• Depositing Lab: Cliff Tabin
• Publication: Burke et al Dev Biol. 1996 Aug 25. 178(1):192-7.

Backbone

• Vector backbone: RCASBP(A)
• Backbone size w/o insert (bp): 11600
• Vector type: Retroviral ; avian expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Hoxc6
• Alt name: Hox C6
• Alt name: Hoxc-6
• Alt name: homeobox c6
• Species: G. gallus (chicken)
• Insert Size (bp): 900
• Entrez Gene: HOXC6 (a.k.a. HOXC-6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: RCAS-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate a retrovirus capable of transcribing Hoxc-6 mRNA, the region encoding the PR11 Hoxc-6 protein was isolated from a chick cDNA clone (Nelson et al., 1996). The PR11 Hoxc-6 protein comprises 235 amino acids (Oliver et al., 1988; Coletta et al., 1991; Shimeld et al., 1993). The ATG of the PR11 coding region was engineered by PCR for cloning into the NcoI site of the SLAX-13 shuttle vector (Riddle et al., 1993), and then subcloned into the retroviral vector RCAS-BP(A) (Hughes et al., 1987). The insert is 932 base pairs and includes approximately 230 bp of 3' untranslated sequence.