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RCAS (A) goosecoid (CT#19)
(Plasmid #13984)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13984 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    goosecoid
  • Alt name
    gsc
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    700
  • Entrez Gene
    GSC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Juan Carlos Ispisua Bel monte

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

gsc ORF ligated into NcoI-Sal I of the Cla 12 Nco adaptor and then cloned into the Cla I site of RCAS (A).

Addgene QC NGS analysis identifies a P2A amino acid discrepancy in the goosecoid insert sequence, relative to NP_990662.2. It is unknown what, if any, impact this may have on plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS (A) goosecoid (CT#19) was a gift from Cliff Tabin (Addgene plasmid # 13984 ; http://n2t.net/addgene:13984 ; RRID:Addgene_13984)

  • For your References section:

    Goosecoid misexpression alters the morphology and Hox gene expression of the developing chick limb bud. Heanue TA, Johnson RL, Izpisua-Belmonte JC, Stern CD, De Robertis EM, Tabin CJ. Mech Dev. 1997 Dec . 69(1-2):31-7. 10.1016/S0925-4773(97)00149-4 PubMed 9486529
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