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Addgene

RCAS (B) flag Radical Fringe (CT#472)
(Plasmid #13983)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCASBP(B)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Radical Fringe
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1200
  • Entrez Gene
    RFNG
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Flag epitope is a Nhe I - BamHI fragment attached to 3' end of radical fringe.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS (B) flag Radical Fringe (CT#472) was a gift from Cliff Tabin (Addgene plasmid # 13983 ; http://n2t.net/addgene:13983 ; RRID:Addgene_13983)

  • For your References section:

    Expression of Radical fringe in limb-bud ectoderm regulates apical ectodermal ridge formation. Laufer E, Dahn R, Orozco OE, Yeo CY, Pisenti J, Henrique D, Abbott UK, Fallon JF, Tabin C. Nature. 1997 Mar 27. 386(6623):366-73. 10.1038/386366a0 PubMed 9121552
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