pRGEB-Cas9-VirD2 (PCO)
(Plasmid
#139802)
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PurposeExpressing Cas9-VirD fusion under ubiquitin promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRGEB32
- Total vector size (bp) 17260
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCas9-VirD2
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SpeciesSynthetic; Plants codon optimized
- Promoter Ubiquitin
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Tag
/ Fusion Protein
- Cas9-VirD2
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer multiple
- 3′ sequencing primer multiple (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRGEB-Cas9-VirD2 (PCO) was a gift from Magdy Mahfouz (Addgene plasmid # 139802 ; http://n2t.net/addgene:139802 ; RRID:Addgene_139802) -
For your References section:
Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice. Ali Z, Shami A, Sedeek K, Kamel R, Alhabsi A, Tehseen M, Hassan N, Butt H, Kababji A, Hamdan SM, Mahfouz MM. Commun Biol. 2020 Jan 23;3(1):44. doi: 10.1038/s42003-020-0768-9. 10.1038/s42003-020-0768-9 PubMed 31974493
Map uploaded by the depositor.
