RCAS (B) flag Lunatic Fringe (CT#471)
(Plasmid
#13980)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13980 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRCASBP(B)
- Backbone size w/o insert (bp) 11600
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Vector typeRetroviral ; avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLunatic Fringe
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SpeciesG. gallus (chicken)
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Insert Size (bp)1200
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Entrez GeneLFNG
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Flag epitope is a Nhe I - BamHI fragment attached to 3' end of lunatic fringe.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS (B) flag Lunatic Fringe (CT#471) was a gift from Cliff Tabin (Addgene plasmid # 13980 ; http://n2t.net/addgene:13980 ; RRID:Addgene_13980) -
For your References section:
Expression of Radical fringe in limb-bud ectoderm regulates apical ectodermal ridge formation. Laufer E, Dahn R, Orozco OE, Yeo CY, Pisenti J, Henrique D, Abbott UK, Fallon JF, Tabin C. Nature. 1997 Mar 27. 386(6623):366-73. 10.1038/386366a0 PubMed 9121552
Map uploaded by the depositor.
