pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)
(Plasmid
#139788)
-
Purpose(Empty Backbone) Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepGEX-2T
-
Vector typeBacterial Expression
-
Tags
/ Fusion Proteins
- GST (cleavable by PreScission Protease or Thrombin) (N terminal on backbone)
- 6xHis (cleavable by TEV protease) (C terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Clone cDNA into vector using standard Restriction Enzyme cloning or using Gibson Assembly Transform verified plasmid into an expression strain (e.g. BL21(DE3)pLysS) for recombinant protein production.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pALS343 (pGEX-2TP-cTevHis-Gibson/MCS) was a gift from Arneet Saltzman (Addgene plasmid # 139788 ; http://n2t.net/addgene:139788 ; RRID:Addgene_139788) -
For your References section:
Multiple Histone Methyl-Lysine Readers Ensure Robust Development and Germline Immortality in Caenorhabditis elegans. Saltzman AL, Soo MW, Aram R, Lee JT. Genetics. 2018 Nov;210(3):907-923. doi: 10.1534/genetics.118.301518. Epub 2018 Sep 5. 10.1534/genetics.118.301518 PubMed 30185429
Map uploaded by the depositor.
