pALS099 (pGEX-2TP-cTevHis-DEST)
(Plasmid #139787)

• Purpose: (Empty Backbone) Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis
• Depositing Labs: Jeannie Lee, Arneet Saltzman
• Publication: Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi: 10.1534/genetics.118.301518. Epub 2018 Sep 5.

Backbone

• Vector backbone: pGEX-2T
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • GST (cleavable by PreScission Protease or Thrombin) (N terminal on backbone)
  • 6xHis (cleavable by TEV protease) (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Grow DEST vector in the presence of AMP and Chloramphenicol
• Copy number: Unknown

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • pALS099_pgex-2tp-tevhis-dest.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Clone cDNA into vector using a Gateway LR reaction. Transform verified plasmid into an expression strain (e.g. BL21(DE3)pLysS) for recombinant protein production.